ABSTRACT
Studies have shown that erythropoietin (EPO) may have neuroprotective effect on cerebral ischemia, and exogenous EPO may also exert neuroprotective effect on cerebral ischemic injury through blood-brain barrier. Its mechanisms may include the upregulation of the expressions of apoptosis inhibiting genes bcl-2 and bcl-xl, the expression of key proteases during the process of inhibiting apoptosis, inhibiting nitric oxide oversynthesis, protecting against glutamate-induced excitotoxicity, etc.
ABSTRACT
Objective To research the expression and correlation between erythropoietin(EPO)mRNA and hypoxia inducible factor-1?(HIF-1?)mRNA in rats with brain ischemic tolerance induced by ischemic preconditioning.Methods Models of cerebral ischemic preconditioning and ischemic-reperfusion(preconditioning group)were made by twice suture method,after the short ischemic preconditioning 1 d,3 d,7 d,14 d and 21 d respectively,the models were underwent ischemic-reperfusion again.The infarct volume was measured by tetrazolium chloride(TTC)staining,the expressions of HIF-1? mRNA and EPO mRNA in the different time(1～21 d)of ischemic-reperfusion were detected by reverse transcriptase polymerase chain reaction(RT-PCR)and compared with non-preconditioning group.Results The infarct volume in the 1 d,3 d and 7 d subgroups of preconditioning group were significantly smaller compared with non-preconditioning group(all P